Re: Egg as a biotin blocker!

From:Connie McManus <conmac@cc.usu.edu>



Amos Brooks wrote:
> 
> Hi,
>     Toss in a slice of bread and it's poached egg with milk toast ... that's
> it ... I'm going on break I'm hungry :-)


Heh!  I'll join you!  I'll take 1/2 bagel and orange juice with a
poached egg.  

Connie M

> Amos Brooks
> ----- Original Message -----
> From: "Patti Loykasek" <ploykasek@phenopath.com>
> To: "histonet" <histonet@pathology.swmed.edu>
> Sent: Monday, May 07, 2001 11:50 AM
> Subject: Re: Egg as a biotin blocker!
> 
> > We use egg substitute that you buy at the grocery. This product is 97-98%
> > egg white. We use 75ml of the egg in 200ml of distilled water. Leave
> slides
> > in for 15 minutes. Wash in 2 changes of distilled water. Put slides in
> skim
> > milk for 15 minutes. Wash in 2 changes of distilled water. Any buffers
> will
> > precipitate out the eggs, so be sure and use water. Dr. Rodney Miller
> > introduced this method of biotin blocking at a workshop I attended. The
> eggs
> > contain avidin, and the milk is a source of biotin. Hope this helps.
> >
> > Patti Loykasek
> >
> >

-- 
ࡱ



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