Re: Egg as a biotin blocker!
|From:||Connie McManus <email@example.com>|
Amos Brooks wrote:
> Toss in a slice of bread and it's poached egg with milk toast ... that's
> it ... I'm going on break I'm hungry :-)
Heh! I'll join you! I'll take 1/2 bagel and orange juice with a
> Amos Brooks
> ----- Original Message -----
> From: "Patti Loykasek" <firstname.lastname@example.org>
> To: "histonet" <email@example.com>
> Sent: Monday, May 07, 2001 11:50 AM
> Subject: Re: Egg as a biotin blocker!
> > We use egg substitute that you buy at the grocery. This product is 97-98%
> > egg white. We use 75ml of the egg in 200ml of distilled water. Leave
> > in for 15 minutes. Wash in 2 changes of distilled water. Put slides in
> > milk for 15 minutes. Wash in 2 changes of distilled water. Any buffers
> > precipitate out the eggs, so be sure and use water. Dr. Rodney Miller
> > introduced this method of biotin blocking at a workshop I attended. The
> > contain avidin, and the milk is a source of biotin. Hope this helps.
> > Patti Loykasek
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