Re: Cyclin D1

From:Luis Chiriboga <>

Hi Donna
We have used cyclin D1, (Zymed & Ventana)  on the nexus.  
The Ventana ab (Thru Cell Marque Cat # 250-2633,  clone DCS-6) works
well with a 20 minute HIER and 32 minute primary incubation.  A good
control is DCIS.
Hope this helps
Regards Luis

"Willis, Donna" wrote:
> Does anyone out there have any ideas on the how to process Cyclin D1 on
> Ventana Nexes, Benchmark or Dako stainers.  We have tried the antibodies
> from several vendors and with varies antigen retrieval solutions and
> methods.
> Thanks,
> Donna Willis
> Histology Lab Manager
> Harris Methodist Fort Worth

Luis Chiriboga Ph.D, H.T. (ASCP) QIHC
New York University School of Medicine
Department Of Pathology 4W27, Bellevue Hospital
27st & First Avenue
New York, NY 10016

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