Re: Astra blue technique

From:"Anthony f. Brandwood" <>

--On 01 May 2001 10:37 -0500 Renee Escalona <> 

> Tony,
> I have never heard of the "Astra blue" technique  but our lab uses a
> Giemsa stain or a Toluidine Blue stain for the demonstration of mast
> cells with much success.  Hope this helps.
> Renee Escalona, BS, HTL(ASCP)
> Laboratory Supervisor
> TX Vet. Med. Diag. Lab.
> College Station, TX

Dear Renee,

Thank you for your reply.We do use Toluidine Blue routinely for mast cells, 
however, one of our Pathologists had found a paper using Asta blue to stain 
the mast cells and then use image analysis to automatically identify them. 
The paper did not give the method for the Astra blue, hence the question on 
the 'Histonet'
Once again many thanks.

Best Wishes,



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