I use osteonectin from U of Iowa Developmental Studies Hybridoma Bank on rat
tissues, mainly bone and cartilage ffpe, formic acid decaled, pre-treatment
is pepsin digestion for 10 min. at 37 dC.
Patsy Ruegg

		-----Original Message-----
		From:	Nperson211@aol.com [mailto:Nperson211@aol.com]
		Sent:	Wednesday, May 02, 2001 10:35 AM
		To:	histonet@pathology.swmed.edu
		Subject:	Osteonectin (SPARC) antibody..help!

		Histonetters,
		Does anyone use an osteonectin antibody reliably in formalin
fixed, paraffin embedded rat tissues (brain)?
		If so, please share info and you will have my undying
gratitude.
		Nancy Lemke
		Henry Ford Hospital
		Detroit, MI