Beth
The following reference to manganese is found in 'Theory and strategy in
histochemistry' edited by Hans Lyon and published by Springer-Verlag.

After treating a section with benzothiazolylazonaphthol, manganese deposits
will stain blue. The method is not specific as other metals, namely cadmium,
zinc and nickel also stain blue. Post-differentiation with 1% oxine
(8-hydroxyquinoline) in 75% ethanol turned tissue cadmium from blue to red
but zinc remained blue.

Regards
Roy Ellis
mailto:roy.ellis@imvs.sa.gov.au


> -----Original Message-----
> From: Histo-Scientific Research Laboratories
> [mailto:histosci@shentel.net]
> Sent: Thursday, 3 May 2001 21:38
> To: Linda McGraf
> Subject: Manganese staining
>
>
> Dear Histonetters,
>
> Has anyone ever heard of staining for localization of manganese in animal
> tissue?  We have looked through our staining books and have come up with
> nothing.  If anyone has ever heard of such a procedure, please share any
> info you may have.
>
> Thank you,
>
> Beth Poole
> HSRL
> beth@hsrl.org
> (540)459-8211
> fax: (540)459-8217
> www.hsrl.org
> 137 South Main Street
> Woodstock, VA  22664
>
>