Charles and interested others:

If you use EGFP (enhanced GFP from Clontech) you can formalin fix, formic
acid decal if needed and paraffin process. The EGFP fluorescent ignal is
easily visible with fluorescent scope or by confocal microscope. Clontech
has extensive information at their website.
Hope that helps, Donna Montague, Center for Orthopaedic Research, UAMS,
Little Rock AR

-----Original Message-----
From: LINDA MARGRAF MD [mailto:LMARGRAF@childmed.dallas.tx.us]
Sent: Monday, May 14, 2001 10:02 AM
To: histonet@pathology.swmed.edu
Subject: GFP


Dear Histonetters; Here's a message from Charles Brown/ Please send replies
to him as I'm not sure if he is on the list. His email is
cbrown@niaid.nih.gov 
Thanks. 


I am currently at the NIH in Bethesda, MD and working with SIV and HIV
research projects.  One of my new areas of research involves the role of
macrophages in the SIV animal model and I wish to tag endosomes with GFP,
inject them IV, and wait for the macrophages to engulf them.  My question is
whether tissue samples from one of these labeled animals can be formalin
fixed, paraffin embedded, sectioned and can the GFP be presered and
localized with basic UV microscopy.  I'm not sure how "stable" the GFP is,
and it would be great to see the directly labeled macs without having to
resort to anti-GFP staining.
As an alternative, I assume samples could be frozen in OCT and the
cryosections should contain the GFP without much interference from tissue
processing.

Thank you for your kind response to these questions,

Sincerely,

Charles Brown
NIH, LMM