Theresa,

The dilution factor was the absolute lowest recommendation quoted on our
data sheets.  The optimal dilution can range from 1:5 to 1:20 depending
on protocol.  The longer you incubate the farther you can take out the
dilution.  This antibody is one of the few that we have that is low
titer.  One option may be to switch to prediluted.

Laura Lewis
Cell Marque Corporation

-----Original Message-----
From: THERESA ROHR [mailto:trohratnyackhospital@hotmail.com]
Sent: Thursday, May 03, 2001 2:01 PM
To: histonet@pathology.swmed.edu
Subject: E-Cadherin


Hello Histonetters,

My Pathologist would like me to get E-Cadherin up and running. Since we
are 
having budget problems, I called around trying to get a free sample.
Cell 
Marque sent me a sample, a .05ml tube. When I called their Tech Service
to 
get a starting titer, they recommended a 1 to 5 dilution. That minute
amount 
not only negates my DAKO Autostainer but would be outrageously expensive
for 
me.  I am using the Envision Plus detection system. Are any of you using

this antibody? What company is your supplier? What is the dilution? Any 
suggestions are welcome.

Thanks,
Theresa Rohr
Section Head, Histology
Nyack Hospital, NY
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