|From:||Laura Lewis <firstname.lastname@example.org>|
The dilution factor was the absolute lowest recommendation quoted on our
data sheets. The optimal dilution can range from 1:5 to 1:20 depending
on protocol. The longer you incubate the farther you can take out the
dilution. This antibody is one of the few that we have that is low
titer. One option may be to switch to prediluted.
Cell Marque Corporation
From: THERESA ROHR [mailto:email@example.com]
Sent: Thursday, May 03, 2001 2:01 PM
My Pathologist would like me to get E-Cadherin up and running. Since we
having budget problems, I called around trying to get a free sample.
Marque sent me a sample, a .05ml tube. When I called their Tech Service
get a starting titer, they recommended a 1 to 5 dilution. That minute
not only negates my DAKO Autostainer but would be outrageously expensive
me. I am using the Envision Plus detection system. Are any of you using
this antibody? What company is your supplier? What is the dilution? Any
suggestions are welcome.
Section Head, Histology
Nyack Hospital, NY
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