RE: Anti-Human Neuroblastoma NB84a from DAKO
|From:||"Nocito, Joseph" <email@example.com>|
we use Dako's NB84, but we use their proteinase K solution (catalog # S3020)
for 10 minutes at room temperature instead of heat retrieval. Then we
perform a 30 minute incubation in the primary, linking and label antibodies
with a 5 minute DAB development. Our titer is 1:3000 and we've had good
results. Hope this helps.
Joe Nocito, B.S., HT(ASCP)QIHC
Christus Santa Rosa & Children's Hospitals
San Antonio, Texas
> -----Original Message-----
> From: Steve Machin UK [SMTP:firstname.lastname@example.org]
> Sent: Friday, May 04, 2001 9:29 AM
> To: Histonet Histonet Histonet
> Subject: Anti-Human Neuroblastoma NB84a from DAKO
> Hello workers,
> Can you help me?
> We have just tried Dako's NB84a (product code M 7075) for neuroblastoma
> can't get it to work.
> The Specification sheet says antigen retrieval by heat works.
> We have tried microwave for 10 mins in the citrate buffer suggested but we
> no staining with dilution as strong as one in 25.
> If anyone out there is using this product can you let us know what are the
> critical factors?
> The previous supplier's, NovoCastra, product worked variably.
> Best Wishes
> Steve Machin UK
> Sheffield Children's Hospital UK
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