Steve,
we use Dako's NB84, but we use their proteinase K solution (catalog # S3020)
for 10 minutes at room temperature instead of heat retrieval.  Then we
perform a 30 minute incubation in the primary, linking and label antibodies
with a 5 minute DAB development.  Our titer is 1:3000 and we've had good
results.  Hope this helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa & Children's Hospitals
San Antonio, Texas 


> -----Original Message-----
> From:	Steve Machin UK [SMTP:stevemachinuk@yahoo.co.uk]
> Sent:	Friday, May 04, 2001 9:29 AM
> To:	Histonet Histonet Histonet
> Subject:	Anti-Human Neuroblastoma NB84a from DAKO
> 
> Hello workers,
> Can you help me?
> We have just tried Dako's NB84a (product code M 7075) for neuroblastoma
> but
> can't get it to work.
> The Specification sheet says antigen retrieval by heat works.
> We have tried microwave for 10 mins in the citrate buffer suggested but we
> get
> no staining with dilution as strong as one in 25.
> If anyone out there is using this product can you let us know what are the
> critical factors?
> The previous supplier's, NovoCastra, product worked variably.
> 
> Best Wishes
> Steve Machin UK
> 
> Sheffield Children's Hospital UK
> 
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