Probe penetration in fixed frozen tissue -Reply

From:Tony Henwood <>

Try fixation in acetic alcohol, after air drying. Follow this with cold
acetone treatment, air dry then apply your probe. For mRNA look for
positive nucleoli.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> Szigeti Csaba <> 11/May/2001 01:57am >>>
Dear members!
Im here again with a question....
I work with frozen spinal cord sections In situ hybridization.
I fix them with 4% paraformaldehid fixative perfusion!
My problem is the probe penetration to the tissue is wery poor!!
How can i dissolve this problem (without the proteinase K treatment).
Can u help me?
Thans a lot for any advise,
All the best for u all!

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