Csaba,
Try fixation in acetic alcohol, after air drying. Follow this with cold
acetone treatment, air dry then apply your probe. For mRNA look for
positive nucleoli.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> Szigeti Csaba <szigcs@bio.u-szeged.hu> 11/May/2001 01:57am >>>
Dear members!
Im here again with a question....
I work with frozen spinal cord sections In situ hybridization.
I fix them with 4% paraformaldehid fixative perfusion!
My problem is the probe penetration to the tissue is wery poor!!
How can i dissolve this problem (without the proteinase K treatment).
Can u help me?
Thans a lot for any advise,
All the best for u all!
Csaba






**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of the
Childrens Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************