Need help with Ki67 mouse
|From:||"Darren Hitchen" <firstname.lastname@example.org>|
I've been trying to get Ki67 in mouse bone to work for some time.
I use 4% Paraformaldehyde in PBS to fix the tissue (overnight)
Decalcification in 125mM EDTA for 2 weeks.
Once embedded and sectioned I've tried heat induced antigen
retrieval with citrate, EDTA, Trypsin. I seem to get no signal
suggesting the pretreatment isn't working, and various degrees of
Has anyone any performed Ki67 staining in mouse tissue?
Is there any other fixative that would work?
Mr D Hitchen,
School of Biological Sciences,
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