Need help with Ki67 mouse
From: | "Darren Hitchen" <mqbssdh2@fs1.scg.man.ac.uk> |
Dear All,
I've been trying to get Ki67 in mouse bone to work for some time.
I use 4% Paraformaldehyde in PBS to fix the tissue (overnight)
Decalcification in 125mM EDTA for 2 weeks.
Once embedded and sectioned I've tried heat induced antigen
retrieval with citrate, EDTA, Trypsin. I seem to get no signal
suggesting the pretreatment isn't working, and various degrees of
background staining?
Has anyone any performed Ki67 staining in mouse tissue?
Is there any other fixative that would work?
Any suggestions
Thanks
Darren Hitchen
Research Technician
Mr D Hitchen,
Room 2.205
School of Biological Sciences,
Oxford Road,
Manchester,
M13 9PT
England.
0161-2756616
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