Hi all!

We have a pathologist who specializes in renal pathology, and our current
arsenal/panel consists of H&E, PAS, and Jones PAMS.  The Jones is a tricky
little bugger. One day it will stain properly and correctly, the next day it
stains only the elastic and tubules in the biopsy, not the glomerular
basement membrane. We've tried troubleshooting this and can find no
correlation to the periodic acid, methenamine, or the silver. Can anyone out
there help us with their experience?

-Teri Johnson
Physicians Reference Laboratory
Overland Park, KS