(Fwd) Re: (Fwd) GFP
From: | "Alice Czarnetzki, AOE" <Czarnetzki.AOE.FAL@kepler.dv.fal.de> |
------- Weitergeleitete Nachricht / Forwarded message -------
Von: "Torsten Thimm" <Thimm.AOE.FAL@kepler.dv.fal.de>
Organisation: FAL
An: "Alice Czarnetzki, AOE" <Czarnetzki.AOE.FAL@kepler.dv.fal.de>
Datum: Tue, 15 May 2001 12:05:46 +0200
Betreff: Re: (Fwd) GFP
Antwort an: torsten.thimm@fal.de
Priorit#228#t: normal
On 15 May 01, at 11:58, Alice Czarnetzki, AOE wrote:
Dear Linda
>
> ------- Weitergeleitete Nachricht / Forwarded message -------
> Datum: Mon, 14 May 2001 10:01:42 -0500
> Von: LINDA MARGRAF MD <LMARGRAF@childmed.dallas.tx.us>
> Betreff: GFP
> An: histonet@pathology.swmed.edu
>
> Dear Histonetters; Here's a message from Charles Brown/ Please send replies to him as I'm not sure if he is on the list. His email is cbrown@niaid.nih.gov
> Thanks.
>
>
> I am currently at the NIH in Bethesda, MD and working with SIV and HIV
> research projects. One of my new areas of research involves the role of
> macrophages in the SIV animal model and I wish to tag endosomes with GFP,
> inject them IV, and wait for the macrophages to engulf them. My question is
> whether tissue samples from one of these labeled animals can be formalin
> fixed, paraffin embedded, sectioned and can the GFP be presered and
> localized with basic UV microscopy. I'm not sure how "stable" the GFP is,
> and it would be great to see the directly labeled macs without having to
> resort to anti-GFP staining.
> As an alternative, I assume samples could be frozen in OCT and the
> cryosections should contain the GFP without much interference from tissue
> processing.
>
I worked with genetically engeneered gfp-tagged marker-genes in
bacteria. to test them in the cryostat, i embedded gfp-marked
bacteria in OCT, but the fluorescence decreased. The best method is
to take a vibratome.
------- Ende der weitergeleiteten Nachricht / End of forwarded message -------
bis bald alice
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