|From:||Brian Chelack <BRIAN.CHELACK@usask.ca>|
I have used ficin in the distant past and found it to be a
very weak enzyme, compared to pronase or trypsin. It was if
I remember correctly it was used mainly by the blood banking
industry. I have an older reference which may of use to you
in the comparison of various enzymatic treatments;
Towle, A.C., Lauder, J.M., and Joh, T.H., Optimization of
tyrosinase hydroxylase immunocytochemistry in poaraffin
sections using pretreatment with proteolytic enzymes, J.
Histochem. Cytochem., 32,766,1984.
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