Ficin comments

From:Brian Chelack <>

Hello Shannon;

I have used ficin in the distant past and found it to be a
very weak enzyme, compared to pronase or trypsin.  It was if
I remember correctly it was used mainly by the blood banking
industry.  I have an older reference which may of use to you
in the comparison of various enzymatic treatments;

Towle, A.C., Lauder, J.M., and Joh, T.H., Optimization of
tyrosinase hydroxylase immunocytochemistry in poaraffin
sections using pretreatment with proteolytic enzymes, J.
Histochem. Cytochem., 32,766,1984.


Brian Chelack
Prairie Diagnostic Services

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