Hello Shannon;

I have used ficin in the distant past and found it to be a
very weak enzyme, compared to pronase or trypsin.  It was if
I remember correctly it was used mainly by the blood banking
industry.  I have an older reference which may of use to you
in the comparison of various enzymatic treatments;

Towle, A.C., Lauder, J.M., and Joh, T.H., Optimization of
tyrosinase hydroxylase immunocytochemistry in poaraffin
sections using pretreatment with proteolytic enzymes, J.
Histochem. Cytochem., 32,766,1984.

regards

Brian Chelack
Prairie Diagnostic Services
Saskatoon