Elastin staining with eNOS staining
From: | Kathie Berghorn <kab35@cornell.edu> |
Dear Histonetters,
I am in need of your collective expertise. We would like to
identify elastin in femoral muscle - stain it and then use a primary
antibody to stain for eNOS. The question is how to stain for elastin
-- our first thought is to use a stain rather than a primary antibody
for this. We heard that Lawson's solution will do this, BUT I do not
have a clue about Lawson's solution. I searched Histonet archives
and Pubmed. Could someone tell me about Lawson's solution?
I also looked in John Kiernan's book and found van Gieson's
method but after reading about it, I think it is more than we need.
I would appreciate suggestions in tackling this. Thank you very much.
Kathie
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