I asked the very same question about one month ago. We are experiencing
this artifact in a variety of ways: ie. special stains (Congo Red, Giemsa,
Fx Muscle stains, Iron stains....decals turn almost a chalky white, and some
ipox.) There are times I can actually see "it" develop and spread before
having to remove and redo the coverslip. Indeed, every now and then I have
to recoverslip some slides 2-3 times! I received advice/suggestions ranging
from not enough xylene on the slides as they run through the coverslipper,
trapped air, to loading only one rack of slides at a time with xylene
dripping from them. We are now going to try putting a little dessicant in
our dehydrating stations. Interestingly, a salesrep for a cover film said
that it seems California is where this phenomenum is happening the most.
Recoverslipping can cause a real time crunch at the most inopportune times!
And recoverslipping 2-3 times for the same slide makes me scratch my head! I
don't recall this kind of problem popping up quite like this and I would love
some real answers. Good luck.....and I hope to read more responses myself.
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