Chromogenic in situ staining with Y-probe

From:Denise Bland-Piontek <>

Hello all:
    Is anyone out there doing any chromogenic in situ staining for the Y
chromosome? I'm getting weak staining and would like any advice from
others using this procedure. I'm using an m-RNA procedure from Biogenex
and I've purchased a biotin labeled Y-probe from ID Labs. Currently I'm
getting variable staining and would appreciate comparing the recipe I
was given from the company with anyone out there who has been doing this
on a regular basis. Thanks in advance,
Denise Bland-Piontek, HTL (ASCP)
University of Minnesota
Anatomic Pathology Research Lab

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