BrdU in Unicryl??

From:Deb Boswell Lane <>

I'm relaying a question from a grad student:
Limulus (horseshoe crab) was embedded in Unicryl and initial attempts at
immunohistochemistry for BrdU haven't worked.  Has anyone done BrdU immuno
work for light microscopy in Unicryl or similar media?  And does anyone
have any suggestions for keeping 10 micron thick paraffin sections of
Limulus (horseshoe crab) tissue on slides through IHC procedures?  Please
make suggestions!  This if for a dissertation?

Thanks in advance!

Deb Boswell Lane
Professional Biomedical Technologist
Biological Imaging
The Jackson Laboratory
600 Main Street
Bar Harbor, Maine 04609
(207) 288-6193

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