Autofluorescent Cells

From:"Plummer, Timothy B." <Plummer.Timothy@mayo.edu>

Hello,

Does anyone know of a technique for blocking the cells in lymphoid specimens
which exhibit intense green (FITC wavelength) autofluorescence.  The
material fluorescing seems to be very granular in appearance and is only
minimally excited by rhodamine exitation-emmission.  I have not taken the
time to identify what the cell type is, but I'm thinking macrophages and I'm
not sure if these cells are naturally autofluorescent AF or have acquired
some AF substance.  I would appreciate any input on this problem, especially
what it might be and if there is a procedure to block it.

Thanks,
Tim




<< Previous Message | Next Message >>