|From:||ANATECH LTD <firstname.lastname@example.org>|
For those of you who would like to try the Astra blue stain for mast
cells but are unable to obtain the dye, use Alcian blue instead.
Make a 1% solution in 0.7 N HCl (or 1 N HCL).
Astra blue is a dye of undisclosed composition, probably closely
related to Alcian blue. Quite possibly the name has been used to
label more than one chemical entity. To my knowledge it is not
available in the USA (at least).
The advantage of using either dye over other cationic (basic) dyes is
that they are not soluble in alcohol, so are not removed during
dehydration. All other blue basic dyes are more soluble in alcohol
than they are in water and thus are easily extracted during
Richard W. Dapson, Ph.D.
Battle Creek, MI
Web address: anatechltdusa.com
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