Hi
I have mouse knees that I am going to decalcify and embed in paraffin so
I can stain them with Saf.O to see GAG content.  I was asked to stain
them with Alizarin Red to see calcium.  Now logic tells me that if I
decalcify these specimens there would be no reason to use Alizarin Red,
and since the mice will be 1 month, 3 months, and 8 months old I'm sure
I would need to decal so I can section them.  Is my logic sound or would
really get some kind of staining even after the calcium is removed?  (I
can't MMA embed because of the Saf.O staining).

Karen Bowden
kbowden@ucsd.edu
UCSD
Ortho Dept.