softening tendon

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From:Rita Angel <>
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I recently read in a histology book that there are procedures before
processing tendon or other hard tissue, that will soften these tissues and
greatly improve the sections you get when cutting.  The book did not give
this protocol, but mentioned using glycerol, alcohol and aniline oil
mixtures.  It also mentionened a brand name of "Millifex".  Does anyone
have a protocol for this type of tissue?  We are sectioning rabbit tendon
and mouse tendon at this time.  We are just soaking longer in an ice/water
mixture, but I would like to get better sections if possible.
Thank you for your help.

Rita Angel

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