---------------------- Forwarded by Kathleen Nagy/ops/diag/sial on
05/17/2000 03:58 PM ---------------------------


"Jeff Crews"<jcrews@organo.com> on 05/17/2000 03:07:34 PM

To:   <knagy@sial.com>
cc:
Subject:  Re[4]: Histogel Question


     I think that Histonet would get you many, many answers to these
     questions, but I'll answer the ones I can. I work in a research lab,
     not a hospital lab, so some things I do very rarely.



1.  Why is acid fast organisms a dark red/purple on tissue and a bright red
on sputum smears, is it due to the tissue thickness?


2.  Do you rinse  tissue stains under lukewarm water or a specific stain
under hot water.   Or does it really matter?


3.  Have you diluted Safranin stain in the Gram Stain?


4.  Why don't you rinse with water after the H & E stain?


5.  After you have placed a coverslip on tissue and placed oil on it,  how
do you clean the slide without removing the coverslip.  Xylene and lens
cleaner seems to leave a film.

6.  Why is Gill 3 both a progressive and regressive stain? Gill 3 is the
most
concentrated of the Gills.


I know that these are very basic questions, but they are so fundamental
that I can't find the answers.  I would greatly appreciate your time and
effort.

Thanks.

Kathy