apoptotic DNA damage detection
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From: | Heike Grabsch <h.grabsch@uni-koeln.de> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi Histonetters,
I am aware that there is the Tunel method to detect apoptotic cells.
REcently I came across an article by Slater M and Murphy CR in The
Histochemical Journal 31:747-749, 1999. They use HRP-conjugated avidin
enhanced with tyramide signal amplification to detect apoptotic DNA
damage. They say that avidin binds with high specifity to
8-oxodeoxyguanosine and to the oxidatively-modified base 8-oxoguanine.
They used it in frozen sections post-fixed with acetone or in another
report post-fixed with 4%PFA.
Anybody out there using this method, too? I am currently wondering
whether this method could be used in paraffin sections, but I am not sure
(I am not a chemist) whether direct tissue fixation with formalin,
processing and paraffin embedding could make you false positives? I
appreciate any comments on this subject.
Dr. Heike Grabsch
Dept of Pathology
University of Duesseldorf
Germany
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