Vector Mouse on Mouse Kit

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From:Emma Carter <E.Carter@OxfordBioMedica.co.uk>
To:"'histonet'" <histonet@pathology.swmed.edu>
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Does anyone have any tips for reducing the background in my sections?
They are mouse tumour cryostat section, fixed in acetone. I used TBS as my
buffer...

There is quite a lot of staining in stroma and the necrotic parts (substrate
= DAB) which, although it doesnt make interpretation impossible, doesnt look
too nice.....

Any ideas?

emma carter



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