Using warm water after Schiffs reagent deepens and develops the color much
better ac/c to Lillie's books.  Also, a slightly warm running water rinse of
10-15 minutes allows hematoxylin to develop and be permanent (meaning NO fading
over time)..sharon osborn

"Bartlett, Jeanine" wrote:

> Sometimes a warm water rinse is used after slides have been in a heated
> staining or silver solution to give them a more subtle cool down.
>
> Jeanine Bartlett
> Centers for Disease Control
> Atlanta, GA
> jbartlett@cdc.gov
>
> -----Original Message-----
> From: Pam Marcum [mailto:pmarcum@polysciences.com]
> Sent: Thursday, May 04, 2000 8:52 AM
> To: Steven D Becker; Histonet@pathology.swmed.edu
> Subject: RE: special stains
>
> I have never used warm or hot water however, we all have superstitions about
> making things work.  Don't know if this one or if it works just with certain
> areas.  Pam Marcum
>
> -----Original Message-----
> From: Steven D Becker [mailto:arnold.5@juno.com]
> Sent: Wednesday, May 03, 2000 10:59 PM
> To: Histonet@pathology.swmed.edu
> Subject: special stains
>
> Does anyone have the reasoning behind people washing the slides in hot
> water after a special stain?  I have always been taught to use cold water
> washes.
>
> Thanks
>
> Jill Stevens
> Kaiser