Re: softening tendon
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Histonet <Histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Wed, 24 May 2000, Barry Rittman wrote:
> what you need is "Mollifex" sold by BDH
You shouldn't use secret mixtures, but Mollifex
isn't (or isn't quite) a secret formulation. BDH
made it following a publication by J. R. Baker
(1941) A fluid for softening tissues embedded in
paraffin wax. Quart. J. Microsc. Sci. 61:75.
Bakers experiments led him to the composition:
Glycerol 10
60% ethyl alcohol: 90
(parts by volume).
A more recent paper is by Maria Wynnchuk (1992)
in J. Histotechnol. 15(4): 321-323. She used
a mixture called Horne's modification of Baker's
solution:
95% ethanol 9520 ml
Glycerol 320 ml
Acetone 80 ml
Liquid phenol 80 ml
(I guess that the "liquid phenol" is an 80%
solution in water, not solid phenol that has
been melted by heating.) 10 litres of softening
solution seems a lot to make up! I wonder
how long it lasts for.
Wynnchuk states that this mixture is better
than Baker's original and that Mollifex, which
contains a "phenol derivative" is just as good.
Anything here for Messrs Sue, Grabbit and Runne?
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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