Re: softening tendon

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From:"Barry Rittman" <>
To:histology <>
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what you need is "Mollifex" sold by BDH

Rita Angel wrote:

> Histonetters,
> I recently read in a histology book that there are procedures before
> processing tendon or other hard tissue, that will soften these tissues and
> greatly improve the sections you get when cutting.  The book did not give
> this protocol, but mentioned using glycerol, alcohol and aniline oil
> mixtures.  It also mentionened a brand name of "Millifex".  Does anyone
> have a protocol for this type of tissue?  We are sectioning rabbit tendon
> and mouse tendon at this time.  We are just soaking longer in an ice/water
> mixture, but I would like to get better sections if possible.
> Thank you for your help.
> Rita Angel

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