---------------------- Forwarded by Rande Kline/EMI/Merck on 05/24/2000 03:01 PM
---------------------------


Rande Kline
05/24/2000 01:04 PM

To:   Rita Angel <angelrj@email.uc.edu>
cc:
Subject:  Re: softening tendon  (Document link: Database 'Rande Kline', View
      '($Sent)')

Rita,

Are you sure of the name?  EM Science and BDH had Mollifex.  The is a reagent
used to quench paraffin embedded tissue blocks.  It would not be used prior to
processing.

Mollifex can be ordered through VWR, 1800-932-5000.  The catalog number is
65039/71, the list price is approximately $23.00 for 500 ml.

Rande Kline, HT (ASCP)
Technical Services
EM Science



Rita Angel <angelrj@email.uc.edu> on 05/24/2000 10:25:35 AM

To:   histonet@pathology.swmed.edu
cc:    (bcc: Rande Kline/EMI/Merck)
Subject:  softening tendon



Histonetters,

I recently read in a histology book that there are procedures before
processing tendon or other hard tissue, that will soften these tissues and
greatly improve the sections you get when cutting.  The book did not give
this protocol, but mentioned using glycerol, alcohol and aniline oil
mixtures.  It also mentionened a brand name of "Millifex".  Does anyone
have a protocol for this type of tissue?  We are sectioning rabbit tendon
and mouse tendon at this time.  We are just soaking longer in an ice/water
mixture, but I would like to get better sections if possible.
Thank you for your help.

Rita Angel