Re: nuclear stain

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>
To:Heike Grabsch <h.grabsch@uni-koeln.de>
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Heike Grabsch wrote:

> Hi Histonetters,
>
> I am sure there is somebody out there who is able to help us! WE do
> double immunhistochemistry labeling, one chromogen is DAB+copper sulfate
> (nively black) for detection of a nuclear antigen, the other is AEC-one
> step solution from Biogenex which gives you a real wonderful red fro a
> cytoplasmic antigen. However, I would like to counterstain all other
> nuclei. I thought about methyl green, however either it is alcohol-based
> like in Romeis (germany histotechnology book) or need to be at least
> dehydrated via n-butanol and xylol linke in John Kiernan's book. But
> alcohol will kill my AEC-staining! Any other green nuclear stain or may
> be yellow which is non-alcohol-based and does not need to be dehydrated?
>

How about dehydrating with acetone or dioxane or tetrahydrofuran or 2.2
dimethoxypropane? Perhaps AEC will survive one of those reagents. Or just
air dry and then go to a clearing agent?

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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