Re: nuclear stain
<< Previous Message | Next Message >>
From: | Jeffrey S Crews <cruzetti@juno.com> |
To: | h.grabsch@uni-koeln.de |
Reply-To: | |
Content-Type: | text/plain |
We use the Polyscientific Light green. Don't have the catalog here but
use it very briefly then rinse the heck out of it in running water. It's
not a nuclear stain but stains everything shades of light green.
Jeffrey Crews, HTL (ASCP)
On Tue, 23 May 2000 20:39:30 -0700 Heike Grabsch <h.grabsch@uni-koeln.de>
writes:
>Hi Histonetters,
>
>I am sure there is somebody out there who is able to help us! WE do
>double immunhistochemistry labeling, one chromogen is DAB+copper
>sulfate
>(nively black) for detection of a nuclear antigen, the other is
>AEC-one
>step solution from Biogenex which gives you a real wonderful red fro a
>
>cytoplasmic antigen. However, I would like to counterstain all other
>nuclei. I thought about methyl green, however either it is
>alcohol-based
>like in Romeis (germany histotechnology book) or need to be at least
>dehydrated via n-butanol and xylol linke in John Kiernan's book. But
>alcohol will kill my AEC-staining! Any other green nuclear stain or
>may
>be yellow which is non-alcohol-based and does not need to be
>dehydrated?
>
>Thanks for your help.
>
>Heike Grabsch
>Dept of Pathology
>University of Duesseldorf
>Germany
>
________________________________________________________________
YOU'RE PAYING TOO MUCH FOR THE INTERNET!
Juno now offers FREE Internet Access!
Try it today - there's no risk! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.
<< Previous Message | Next Message >>