Re: nuclear stain

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From:Jeffrey S Crews <>

We use the Polyscientific Light green. Don't have the catalog here but
use it very briefly then rinse the heck out of it in running water. It's
not a nuclear stain but stains everything shades of light green.

Jeffrey Crews, HTL (ASCP)

On Tue, 23 May 2000 20:39:30 -0700 Heike Grabsch <>
>Hi Histonetters,
>I am sure there is somebody out there who is able to help us! WE do 
>double immunhistochemistry labeling, one chromogen is DAB+copper 
>(nively black) for detection of a nuclear antigen, the other is 
>step solution from Biogenex which gives you a real wonderful red fro a 
>cytoplasmic antigen. However, I would like to counterstain all other 
>nuclei. I thought about methyl green, however either it is 
>like in Romeis (germany histotechnology book) or need to be at least 
>dehydrated via n-butanol and xylol linke in John Kiernan's book. But 
>alcohol will kill my AEC-staining! Any other green nuclear stain or 
>be yellow which is non-alcohol-based and does not need to be 
>Thanks for your help.
>Heike Grabsch
>Dept of Pathology
>University of Duesseldorf

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