Hi Heike

We have just started to use rb-a-c-kit from Santa Cruz (sc-168) on paraffin
sections of GIST (gastrointestinal stroma tumors), where it works nicely.
Dilution is 1:50, pretreatment in 10 mM citrate buffer, pH 6.0, pressure
cooker, with 7 min under full pressure. My first impression with bone marrow
trephines, however, is mixed: we do barely see any c-kit positive stem
cells, but may be our staining conditions are not (yet) optimized for this
type of tissue / cells.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland