Sonia,

i use the DAKO biotin blocking in conjunction with DAKO LSAB peroxidase
kit.  I use this to stain HBV in transgenic mice.  According to the
instructions I was given by the DAKO people, the biotin blocking kit is
used first thing before any other reagents. 

Connie McManus
 




At 02:39 PM 05/18/2000 +0000, Sonia Greco wrote:
>Dear Histonetters,
>
>I just bought the DAKO Biotin Blocking System, which suggests to use before 
>application of the first step of the staining procedure. Does this mean 
>after the secondary antibody, before a strepABC and DAB staining. or do I 
>apply it before between the primary and secondary antibodies?
>
>Also: I am doing immunohistochemistry with a polyclonal goat anti human 
>antibody. For a negative control, I would have liked to use a goat 
>non-immune IgG, but didn't have any, so I have used normal goat sera. Would 
>you know the best dilution to use to make it a good negative control?
>
>Thanks in advance
>
>Sonia
>QIMR, Australia
>
>
>
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA