Re: biotin blocking and non-immune sera for negative control

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From:Connie McManus <>
To:Sonia Greco <>, "'HistoNet Server'" <>
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i use the DAKO biotin blocking in conjunction with DAKO LSAB peroxidase
kit.  I use this to stain HBV in transgenic mice.  According to the
instructions I was given by the DAKO people, the biotin blocking kit is
used first thing before any other reagents. 

Connie McManus

At 02:39 PM 05/18/2000 +0000, Sonia Greco wrote:
>Dear Histonetters,
>I just bought the DAKO Biotin Blocking System, which suggests to use before 
>application of the first step of the staining procedure. Does this mean 
>after the secondary antibody, before a strepABC and DAB staining. or do I 
>apply it before between the primary and secondary antibodies?
>Also: I am doing immunohistochemistry with a polyclonal goat anti human 
>antibody. For a negative control, I would have liked to use a goat 
>non-immune IgG, but didn't have any, so I have used normal goat sera. Would 
>you know the best dilution to use to make it a good negative control?
>Thanks in advance
>QIMR, Australia
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT

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