Re: Tyramide amplification
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | "Sherring, Yoshika" <SherriY@nenlifesci.com> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Tue, 16 May 2000, Sherring, Yoshika wrote:
> I understand that there are publications stating how to make your own tyramide.
> ... these publications are infringing upon NEN's patents ...
This has to be complete rot. The first publication I know of that
gives instructions for doing tyramine conjugation is in 1989, long
before the publication of histochemical applications of these
reagents, and surely also before anyone thought of trying to
patent the simple benchtop procedure. The authors of the 1989 paper
(which may not be the first) must have got their chemical know-how
from somewhere, too. The use of succinimide esters to join molecules
together has been common knowledge and standard practice for many
years. It's in the textbooks. Also, just look at any catalogue of
the Pierce company (just one example) during the last 25 years.
They have been selling all kinds of reagents (and kits) for linking
biotin and other compounds to amines and proteins.
To say that it's illegal for anyone to make tagged tyrosine is
equivalent to saying you can't to make your own wallpaper
glue out of flour and water when more expensive patented
adhesives are available in the shops.
The only effect of these aggressively protective messages will be
to discourage scientists from buying anything at all from NEN.
We'll spend our money with companies that don't threaten to sue
their potential customers.
John A. Kiernan, MB, ChB, PhD, DSc,
Professor, Dept of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
FAX (Department): (519) 661-3936
E-mail: kiernan@uwo.ca
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