Patsy, and all histonetters,
Last time I cheked, it is not a crime to give out a referenced article.

The most recent literature I have found on Tyramide is:

Title: Tyramine- Amplified Immunohistochemical Testing Using "Homemade"
Biotinylated Tyramine Is Highly Sensitive and Cost-Effective

Jacobs W, Dhaene K, Van Marck E, Tyramine-Amplified Immunohistochemical
Testing Using "Homemade" Biotinylated Tyramine Is Highly Sensitive and
Cost Effective. Arch Pathol Lab Med - Vol 122, July 1998; 122:642-643.


Remember you cannot try this in your Lab, it just makes interesting
reading material. Please let me know of any other articles that you guys
may know about. I am interested in thias subject.

M. Price
On Mon, 22 May 2000 08:40:33 -0600 rueggp <rueggp@earthlink.net> writes:
> Marsha,
> My advise to you is to give out the references, surely that can be 
> no crime.
> Patsy
> 
> marsha r price wrote:
> 
> > Patsy,
> > I agree with you.
> >
> > I have had an overwhelming response from histonetters, that were
> > interested in the Homemade Tyramide. I wanted all to know that I 
> am not
> > ignoring your requests.
> >
> > There are some interesting recent articles out though that tells 
> you how
> > to make your own, but according to the folks at NEN, even if you 
> come
> > across one of those articles, it is infringement for you to follow 
> the
> > instructions and make your own and do your own research without
> > permission and a license from them to even be able to use it in 
> your lab.
> >
> > Again! I apologize for the mix-up and I am not ignoring your 
> e-mails, I
> > just have my hands tied so to speak and cannot give this recipe 
> out
> > because of legal reasons.
> >
> > Marsha Price
> >
> > On Fri, 19 May 2000 20:31:44 -0600 rueggp <rueggp@earthlink.net> 
> writes:
> > > well, well, pretty soon you won't be able to make up your own 
> PBS or
> > > Tris
> > > buffer, sounds like a crock to me, just like biogenex having the
> > > market on
> > > unmasking antigens using heat a few years back.
> > > patsy ruegg
> > >
> > > "marsha r price (by way of Histonet)" wrote:
> > >
> > > > Matthew,
> > > > I guess no one received my message earlier. Shortly after I
> > > offered to
> > > > give out the recipe for Homemade Biotinylated Tyramide, I 
> received
> > > a
> > > > threatning letter from NEN LifeScience explaining to me that 
> they
> > > have an
> > > > exclusive patent on tyramide and they are the only ones who 
> can
> > > buy sell
> > > > or make Tyramide.
> > > >
> > > > I sent an e-mail out to the histonet, explaining that I would 
> not
> > > be able
> > > > to give that recipe out because of possible legal 
> ramifications
> > > that
> > > > could be sanctioned against me.
> > > >
> > > > Again I apologize I was unaware that NEN had the exclusive 
> patent
> > > on
> > > > Tyramide. I am not that familiar with patent laws, I thought 
> as
> > > long as
> > > > you did not use their recipe and did not sell it, you could 
> make
> > > your
> > > > own. Kind of like the sausage control that has a patent. I 
> thought
> > > > everyone was making their own and you would have to have
> > > permission to
> > > > sell it from the person who has the patent. I am talking to a
> > > Patent
> > > > Lawyer today because I may possibly patent one of my 
> procedures of
> > > > skipping the deparrafinization step in Immunos.
> > > >
> > > > According to NEN, we can't even make our own Tyramide and use 
> it
> > > in our
> > > > labs at our own discretion, we have to have permission or a
> > > license to
> > > > enen use it. I will forward you a copy of their letter also.
> > > >
> > > > M.Price
> > >
> >
> > ________________________________________________________________
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> 

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