Re: Tyramide amplification- Recipe for Homemade Tyramide

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From:marsha r price <> (by way of Histonet)
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I guess no one received my message earlier. Shortly after I offered to
give out the recipe for Homemade Biotinylated Tyramide, I received a
threatning letter from NEN LifeScience explaining to me that they have an
exclusive patent on tyramide and they are the only ones who can buy sell
or make Tyramide.

I sent an e-mail out to the histonet, explaining that I would not be able
to give that recipe out because of possible legal ramifications that
could be sanctioned against me.

Again I apologize I was unaware that NEN had the exclusive patent on
Tyramide. I am not that familiar with patent laws, I thought as long as
you did not use their recipe and did not sell it, you could make your
own. Kind of like the sausage control that has a patent. I thought
everyone was making their own and you would have to have permission to
sell it from the person who has the patent. I am talking to a Patent
Lawyer today because I may possibly patent one of my procedures of
skipping the deparrafinization step in Immunos.

According to NEN, we can't even make our own Tyramide and use it in our
labs at our own discretion, we have to have permission or a license to
enen use it. I will forward you a copy of their letter also.


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