>Date: Sun, 14 May 2000 15:25:22 -0700
>From: Heike Grabsch <h.grabsch@uni-koeln.de>
>X-Mailer: Mozilla 2.01 (Win95; I; 16bit)
>To: "Jill McVee (by way of Histonet)" <jm36@st-andrews.ac.uk>
>CC: HistoNet@pathology.swmed.edu
>Subject: Re: Proliferating cell markers
>
>Hi Jill,
>
>is there a reason why you do not want to use an antibody against the
>Ki-67 protein? I thinks that there is a clon which works in mouse. In
>general the clone name for a reliable Ki-67 antibody is "MIB", so there
>is MIB1, which works in human paraffin sections, sold by Dianova. I am at
>home at the moment so I cannot look into the Dianova catalog, but I am
>rather sure, there is a MIB clone, which works in mouse. However Ki-67
>is a general proliferation marker, meaning it is only negative if cells
>are in G0-phase, and it is positive in alle phases of the cell cycle, but
>it was published that the staining correlates with anti-BrdU
>immunhistochemistry.
>There is an excellent review about the Ki-67 protein just published: by
>Scholzen T and Gerdes J: The Ki-67 Protein: from the known and the
>unknown in J Cell Physiol 182:311-322, 2000. You can contact the author
>and ask for a reprint as I did via tscholzen@fz-borstel.de
>
>I hope this helps,
>
>Dr. Heike Grabsch
>Dept of Pathology
>University of Duesseldorf
>Germany
>
>Jill McVee (by way of Histonet) wrote:
>>
>> Hi histonetters,
>>                  I have a Research Fellow who is in need of your global
>> expertise. She would like to know if anyone has a proliferating cell marker
>> anti-body
>> which they use routinely  in  IC and is reliable. At the moment she is
>> using anti-Brdu in mouse neuronal tissue  after in vivo injection but would
>> like to change to a less invasive method of detecting proliferating cells.
>> So if anybody out there has replaced their Brdu with another method please
>> let me know.
>> Jill McVee
>> Histologist
>> St.Andrews Uni
>> Fife. Scotland.
>
>