Re: Nonspecific staining with rabbit neg control

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From:DDittus787@aol.com
To:vlm_23@n2mail.com, histonet@pathology.swmed.edu
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Dear Vicki
Most tissue has endogenous biotin, but liver has the most, try a 
pre-treatment before staining of 3% H2O2 for 10 min at room temp, it may 
help, also Ventana has a biotin bloking kit you can add on line.             
Dana



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