Re: Her-2-Neu

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From:Luis Chiriboga <>
To:Jerry Santiago <>
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Use the ventana her-2-neu (CB11) with a 10 minute 0.01M pH6.0 citrate microwave,  32 minute incubation on the nexus stainer.
Use the zymed her-2-neu (TAB250) 1:20 overnight on the nexus stainer.  NOt sure if this is the V-line antibody.
recently did a comparison among erb antibodies.  abstract presented at CAP meeting.
Titled "Variability of Her-2/neu detection in archival breast carcinoma using clones CB11, AO485, TAB250 and 3B5 and different grading schemes"

Jerry Santiago wrote:

> We are doing a comparison study with Her-2-Neu, and I will like to know if anybody have experience with the V-Line Her-2-Neu  from Zymed, and/or the  Ventana Ready Her-2-Neu?
> Thanks

Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016

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