Crescent Manufacturing makes the blades and can be contacted at
www.crescentblades.com.  Also  you can reach Crescent by going to the histotech's
home page provided by Steven Slap and  Peggy Wenk at www.histology.to.

RDavenport@stmarygj.com wrote:

> Hi,
> does anybody know who  makes DURA EDGE  microtome blades?
>
> Renate
> St. Mary's Hospital , Grand Junction, Co 81506
>
> >----------
> >From:  HistoNet Server[SMTP:histonet@pathology.swmed.edu]
> >Sent:  Sunday, May 14, 2000 11:05 PM
> >To:    HistoNet Server
> >Subject:       Daily Digest
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:47:12 -0500
> >From: Katri Tuomala <katri@istar.ca> (by way of Histonet)
> >Subject: Test
> >
> >Test
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:47:31 -0500
> >From: NLOUISEA@aol.com (by way of Histonet)
> >Subject: testing
> >
> >testing
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:47:51 -0500
> >From: "Eugene J. McCoy" <eugene.j.mccoy@worldnet.att.net> (by way of
> >Histonet)
> >Subject: Helicobacter Pyloric Stain
> >
> >Coni Forney  I Faxed you the procedure to do the best H. pyloric stain you
> >will ever see.  At the bottom  of the page I put my phone number, if you
> >need a little help with the procedure give me a call and I will help you
> >enjoy the stain.  P.S. don't for get to make your Acidulated water first,
> >its easy.
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:48:06 -0500
> >From: ToniT41@aol.com (by way of Histonet)
> >Subject: Halt in the water bath!!
> >
> >I have recently tried a solution called Halt.  It really helps get out the
> >wrinkles....If any one wants to try it I got it from PolyScientific....They
> >will send you a sample if you ask...
> >I also haven't had any problems with any tissue washing off...I personally
> >think the stuff is great...
> >
> >Toni
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:48:20 -0500
> >From: ToniT41@aol.com (by way of Histonet)
> >Subject: Alcian Yellow references
> >
> >Does anyone have any references for Alcian Yellow? They can be in Journals,
> >or books.  Something that I can show my pathologist....geez...he drives me
> >crazy!!!
> >
> >Thanks,
> >Toni
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:48:43 -0500
> >From: "ANN MARUSKA" <amarusk1@FAIRVIEW.ORG> (by way of Histonet)
> >Subject: in situ
> >
> >Hi fellow Histonetters,
> >
> >Does anyone out there have experience with the instrument Vysis, used for
> >FISH?  If you have any comments you would like to share, I would greatly
> >appreciate it - and it will be kept completely confidential.
> >TIA.
> >
> >Ann Maruska
> >IHC Pathology
> >Fairview-University Med Ctr.
> >2414 South 7th Street
> >Mpls. MN 55454
> >612-672-4005
> >amarusk1@fairview.org
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:48:58 -0500
> >From: "Garza-Williams, Sara" <Garza-Williams.Sara@tchden.org> (by way of
> >Histonet)
> >Subject: Clothing etiquette for the bench tech.
> >
> >
> >
> >Please, please I need the your help regarding my argument to a CAP
> >inspector (lab general).
> >
> >
> >She gave me a huge "Ding" regarding the fact that our techs were not
> >wearing lab coats(in the cutting and staining area), they do wear gloves.
> >She also justified her point by indicating because the techs were wearing
> >jeans it was "unprofessional".  Of course, I could hardly contain myself
> >and had try very hard to control myself from stating what I really wanted
> >to say...
> >
> >
> >I did tell her,  specimens were never triage in the main lab, so full PPE
> >was irrelevant.  Our techs do wear nitrile gloves for protection from the
> >reagents and chemicals.
> >
> >
> >She said full PPE was essential because the blocks and slides were
> >considered infectious. So of course I said "At what point does the slide
> >and/or block ever become non-infectious-when it reaches the pathologist?"
> >She said "They should continue to be considered infectious and that the
> >pathologist should be wearing lab coats when they read out slides."  I said
> >"Should the pathologist wear gloves as well, they have to touch the
> >slide".  Our exchange went on and on until she said "I have to give you the
> >deficiency".
> >
> >
> >My pathologist went ballistic, as I did.  He and I would like to know what
> >do other labs do?
> >We are determined to argue for principle sake only.  My pathologist feels
> >that PPE is essential when you consider every situation with logic but he
> >doesn't care if the techs cut naked (metaphorically). in the lab because
> >they do such good work and work safe.
> >
> >
> >So my question is,  Do you require techs to wear a lab coat in the main
> >part of the lab?  What is your lab policy?  Our policy (approved by the
> >Infectious Disease department)  specifically states that no PPE is required
> >in the main lab area, but full PPE is required in the gross room ,morgue or
> >when cutting frozen sections.
> >
> >
> >Of the hospitals that I've seen (a least a dozen) none of them require
> >their techs to wear coats.
> >I'd love the hear from other labs.  Sorry about the long story but I'm
> >still steaming....
> >
> >
> >Thanks
> >Sara
> >
> >
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:49:13 -0500
> >From: Connie McManus <conmac@cc.usu.edu> (by way of Histonet)
> >Subject: Warthin Starry (was h. pylori stain)
> >
> >At 10:57 AM 05/11/2000 +0200, Buttigieg Carmen at MOH wrote:
> >>Dear Dana
> >>
> >>Is it possible to have a copy of this technique + catalogue numbers of the
> >>reagents. We currently use the Warthin Starry. Results are good but the
> >>technique is long.
> >
> >
> >I don't find the Warthin Starry to be a long stain to do.  What is your
> >procedure?  Mine is very simple and I always get great results with it.
> >
> >
> >
> >Connie McManus
> >Veterinary Diagnostics Lab
> >Utah State University
> >Logan, UT
> >USA
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:49:33 -0500
> >From: Jig1357@aol.com (by way of Histonet)
> >Subject: Re: PAS DIASTASE
> >
> >We use diastase of malt, from Fisher.  I can get the catalogue number if you
> >are interested.  .5 grams in 100 ml distilled water. Treat slides for 20 min.
> > Another related question concerning diastase:
> > I have worked several places and some will preheat the diastase solution to
> >37 C then incubate the slides at that temp for 20-30 min.  Others just use
> >the solution at room temp.   What are you folks doing for this procedure?
> >Thanks!
> >
> >Jeanne Godine
> >Pathology Consultants
> >Lynchburg VA
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:49:49 -0500
> >From: Amos Brooks <atbrooks@snet.net> (by way of Histonet)
> >Subject: Re: Slide Adhesive for Bone
> >
> >Hi,
> >    Don't use a microwave. At least an hour in a 60 degree oven should do the
> >trick. Enzymatic predigestion for antigen retrieval is usually harsh. If
> >you are
> >using this try something different. 3% H2O2 is also harsh on many tissues try
> >.03% for a longer time. If any tissue has survived your heating steps these
> >suggestions may help keep it on the slides.
> >Good Luck
> >Amos Brooks
> >
> >Jay Turner wrote:
> >
> >> I've been having a lot of trouble with sections lifting during the heating
> >> steps of anitgen retrieval for IHC stains on bone samples.  There isn't any
> >> problem with the surrounding soft tissue (i.e. muscle), but the cortical
> >> bone refuses to stay on the slide.  I've tried using Plus slides, coating
> >> with poly-L-lysine, casein glue...almost everything that's out there.
> >>
> >> Does anyone have any suggestions for either an alternative slide adhesive
> >>or
> >> an alternative step that would be just as effective as HIER in bone?
> >>
> >> Thanks in advance.
> >>
> >> Jay
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:50:05 -0500
> >From: Atoska Gentry <gentras@vetmed.auburn.edu> (by way of Histonet)
> >Subject: ASH Spring Seminar
> >
> >       Rita Humphries or Larry Parks I need to talk to you about the ASH
> >Spring
> >Seminar  registration please contact me ASAP.  Thanks,  Atoska
> >
> >Atoska  S. Gentry B.S., HT(ASCP)
> >Research Assistant II
> >Scott-Ritchey RSCH Center
> >Auburn University, AL  36849
> >PH:   (334) 844-5579
> >Fax:  (334) 844-5850
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:50:20 -0500
> >From: "Horn, Hazel" <HornHazelV@exchange.ach.uams.edu> (by way of Histonet)
> >Subject: RE: CPT code for Hpylori screens
> >
> >> We use CPT 87072 for the CLO Test   for h. pylori
> >[Horn, Hazel]
> >> -----Original Message-----
> >> From:        MacDonald, Jennifer [SMTP:jmacdonald@sach.org]
> >> Sent:        Thursday, May 04, 2000 03:57 PM
> >> To:  'Histonet'; 'Goodwin, Diana'
> >> Subject:     RE: CPT code for Hpylori screens
> >>
> >> Our pathologist charge an 88300 for a gross only.
> >>
> >> > ----------
> >> > From:      Goodwin, Diana[SMTP:DGoodwin@CHSNJ.org]
> >> > Sent:      Thursday, May 04, 2000 12:00 PM
> >> > To:        'Histonet'
> >> > Subject:   CPT code for Hpylori screens
> >> >
> >> > Hello, Histonetters.
> >> >
> >> > The weather here on the east coast, USA, has FINALLY gotten Spring-like
> >> > and  we are enjoying a long-awaited drenching of sunshine and mild
> >> > temperatures.
> >> >
> >> > Does anyone know if a CPT code exists for the reading and reporting of
> >> > agar-type urease screen tests for H pylori, such as Clo-Test and hpFast,
> >> > and also how to document these in workload tallies?
> >> >
> >> > Thanks in advance,
> >> >
> >> >
> >> > Diana Goodwin,  HT
> >> > Trenton,  NJ
> >> >
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:50:39 -0500
> >From: Anna Logvinova <alogvinova@buckcenter.org> (by way of Histonet)
> >Subject: No mail today?
> >
> >Strangely I stopped recieving Histonet mail exchange today. Is there
> >anything wrong with the server?
> >Otherwise, please check my subscription. I miss it!
> >
> >Thanks,
> >
> >Anna
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:50:54 -0500
> >From: RetTek2000@aol.com (by way of Histonet)
> >Subject: TEST......2PM
> >
> >
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:51:11 -0500
> >From: RetTek2000@aol.com (by way of Histonet)
> >Subject: NO MESSAGES RECEIVED
> >
> >Are you down today. No messages are being received..........
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:51:26 -0500
> >From: Greg Dobbin <dobbin@Upei.CA> (by way of Histonet)
> >Subject: Hep. Growth Factor
> >
> >Hi All (again),
> >
> >I need to find an antibody to hepatocyte growth factor (HGF) that will
> >work in FFPE tissues. Any suggestions?
> >
> >I will be staining canine livers. Anything to add? Any and all help
> >greatly appreciated.
> >
> >Cheers! Greg
> >
> >>
> >>
> >>
> >>
> >>
> >>
> >Greg Dobbin
> >Pathology Lab
> >Atlantic Veterinary College, U.P.E.I.
> >550 Unviversity Ave.
> >Charlottetown, P.E.I.
> >C1A 4P3
> >Phone: (902)566-0744
> >Fax: (902)566-0851
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:51:42 -0500
> >From: Greg Dobbin <dobbin@Upei.CA> (by way of Histonet)
> >Subject: Tyramide amplification
> >
> >Hi All,
> >Could someone provide me with a brief explanation of the tyramide
> >amplification technique and perhaps a reference or two, that would
> >describe the method.
> >
> >I seem to recall there were patent problems/issues with copying the
> >method. Has that all been sorted out? Is there a kit now comercially
> >available?
> >Cheers!  Greg
> >>
> >>
> >>
> >>
> >>
> >>
> >Greg Dobbin
> >Pathology Lab
> >Atlantic Veterinary College, U.P.E.I.
> >550 Unviversity Ave.
> >Charlottetown, P.E.I.
> >C1A 4P3
> >Phone: (902)566-0744
> >Fax: (902)566-0851
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:52:00 -0500
> >From: stephen asquith <stephen.asquith@kcl.ac.uk> (by way of Histonet)
> >Subject: anti-rat beta APP
> >
> >Dear all
> >we have been using anti-rat beta APP from Boehringer on fixed frozen tissue
> >with only limited results. Most of the work in the literature uses antigen
> >retrival on paraffin sections.
> >1.Is there a reason why the the antibody shouldn't work on the frozen tissue?
> >2.Does anyone have another APP antibody that gives good cositant results.
> >Any suggestions or comments greatly welcomed
> >Thanks again.
> >Steve
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:52:16 -0500
> >From: taffy <hooser@anatomy.iupui.edu> (by way of Histonet)
> >Subject: Platoid N
> >
> >Hello..AGAIN
> >Does anyone have ANY idea as to a supplier for Plastoid N?  ANY LEADS ?
> >Once more,
> >THANKS
> >Taffy Hooser
> >
> >
> >
> >
> >
> >Mary (Taffy) Hooser,  HT (ASCP)
> >Indiana University School of Medicine
> >Dept. of Anatomy and Cell Biology
> >635 Barnhill Dr.  MS 5045 G
> >Indianapolis, IN  46202
> >
> >Phone:  (317)274-7558
> >Fax     : (317)278-2040
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:52:31 -0500
> >From: Jill McVee <jm36@st-andrews.ac.uk> (by way of Histonet)
> >Subject: Proliferating cell markers
> >
> >Hi histonetters,
> >                 I have a Research Fellow who is in need of your global
> >expertise. She would like to know if anyone has a proliferating cell marker
> >anti-body
> >which they use routinely  in  IC and is reliable. At the moment she is
> >using anti-Brdu in mouse neuronal tissue  after in vivo injection but would
> >like to change to a less invasive method of detecting proliferating cells.
> >So if anybody out there has replaced their Brdu with another method please
> >let me know.
> >Jill McVee
> >Histologist
> >St.Andrews Uni
> >Fife. Scotland.
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:53:02 -0500
> >From: "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of Histonet)
> >Subject: Re: PAS DIASTASE etc
> >
> >On Thu, 11 May 2000 Eileen_Dusek@cdh.org wrote:
> >
> >> Does anyone have a procedure for Pas Diastase without using saliva.  In
> >> this day and age of safety i am surprised we still use our own saliva.
> >
> >  In the absence of some active infection of the mouth, such as
> >  streptococci, herpes simplex or Vincent's angina, it isn't easy
> >  to think of saliva as unsafe. Even if you're a carrier of
> >  meningococci or something potentially harmful to some others,
> >  putting a drop of spit on a slide isn't exactly a promiscuous
> >  and unregulated kiss-a-thon. For places that are short of cash,
> >  such as hospitals in the middle of Africa and research laboratories
> >  in Canada, saliva is a free, safe and very practical source of
> >  the enzyme amylase (= diastase).
> >
> >  The arguments for using amylase from a chemical supplier are,
> >  despite the truths in the preceding paragraph, quite strong.
> >
> >  (1) It isn't expensive (even in Canada) unless for some reason you
> >      need some particular highly purified variant of the enzyme.
> >      For confirming starch or glycogen, you don't.
> >  (2) With bought amylase you can use a higher concentration than
> >      your own salivary glands could ever put out, and consequently
> >      the time to digest glycogen is shorter.
> >  (3) Amylase isn't the only enzyme in spit. Proteolytic enzymes,
> >      lysozyme and ribonuclease are other well known ingredients,
> >      and there are surely many more. After all, this is the liquid
> >      that cleaned people's teeth before the invention of the
> >      toothbrush (except in India and perhaps elsewhere, where they
> >      are lucky enough to have trees with suitably bristly twigs).
> >
> >  The other enzymes in saliva are unlikely to catalyze the hydrolysis
> >  of substances in the section that would be stained by a method to
> >  show glygogen (such as PAS or Best's carmine).
> >
> >  Instructions for making your own salivary RNase are in the 2nd
> >  edition of Pearse's Histochemistry (1960). You heat to 90C for
> >  a while, which inactivates all the other enzymes. I did this
> >  once, years ago, and it worked: prevented the red staining of
> >  neuronal RNA (Nissl substance) by methyl green-pyronine.
> >
> > John A. Kiernan,
> > Department of Anatomy & Cell Biology,
> > The University of Western Ontario,
> > LONDON,  Canada  N6A 5C1
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:53:39 -0500
> >From: TWARE4U@aol.com (by way of Histonet)
> >Subject: Re: PAS DIASTASE
> >
> >Dear Eileen,
> >We use Poly Scientific's buffer and amylase sets. Unfortunately I am
> >currently on maternity leave and I do not have the catalog numbers for you.
> >If you have there catalog or if anyone out there can help I'd appreciate it.
> >You don't have to use saliva it takes a little longer ,unless you have a
> >microwave to speed things up a bit. I hope I helped you.
> >Lisa
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:53:57 -0500
> >From: Brown Alex <Alex.Brown@aaaht.scot.nhs.uk> (by way of Histonet)
> >Subject: RE: h.pylori stain
> >
> >Hi Coni,
> >       We use a modified Cresyl Fast Violet. We came across the method in a
> >journal or paper a few years ago, unfortunately though the reference for it
> >disappeared a long time ago.  ( If anyone recognises this technique I would
> >appreciate the reference for it )
> >Hope it's not subject to Copyright :#194#)
> >
> >
> > Method :
> >
> > 1) Sections to water
> > 2) Cresyl Violet solution                     5 mins
> > 3) Rinse in water
> > 4) Rinse in 95% alcohol ( ethyl or methyl )
> > 5) Differentiate in Cresyl Violet Differentiator until -
> >       Nuclei          Violet
> >       Cytoplasm       Almost colourless
> >       Organisms       Deep Blue - Violet
> > 6) Rinse in absolute alcohol
> > 7) Clear and mount.
> >
> >Solutions :
> >
> >Cresyl Fast Violet -   0.2% Cresyl Fast Violet Acetate in distilled water
> >
> >Differentiator          -              95% alcohol             90 ml
> >                       Chloroform              10 ml
> >                       Glacial Acetic Acid       3 drops ( we use a
> >disposable Pastette, so it's approx 0.15 ml )
> >
> >       Alex Brown
> >       Crosshouse Hospital
> >       Kilmarnock,Scotland.
> >
> > ----------
> >From: Forney Constance M Civ 74 MDSS/SGSC
> >To: 'HISTONET'
> >Subject: h.pylori stain
> >Date: Wednesday, May 10, 2000 6:12PM
> >
> >Looking for a quick and easy (ha ha) but excellent stain for h. pylori.
> >
> >Thanks,
> >
> >Coni Forney, BS MT,HT(ASCP)
> >Technical Supervisor , Histopathology
> >USAF Medical Center
> >Wright Patterson, AFB, OH 45433
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:54:17 -0500
> >From: terij@prlnet.com (Teri Johnson) (by way of Histonet)
> >Subject: Re: PAS DIASTASE
> >
> >We use powdered Diastase and phosphate buffer from Rowley Biochemical.  It
> >works quite well, especially in light of the fact we do so many of them.
> >Just dissolve 0.1 g of Diastase in 100 ml of the buffer (pH 6.0).  Preheat
> >to 37 degrees C, and place in the solution for one hour.  Rinse, and proceed
> >with the PAS reaction.
> >
> >The catalog numbers are:  Diastase E-340-1, and Phosphate buffer, pH 6.0
> >E-340-2.
> >
> >Good luck to you!
> >
> >- -Teri
> >- ----- Original Message -----
> >From: <Eileen_Dusek@cdh.org>
> >To: <HistoNet@pathology.swmed.edu>
> >Sent: Thursday, May 11, 2000 11:21 AM
> >Subject: PAS DIASTASE
> >
> >
> >> Hello everyone,
> >> Does anyone have a procedure for Pas Diastase without using saliva.  In
> >> this day and age of safety i am surprised we still use our own saliva.
> >> I know Sigma has an Amylase Type IX-A, is anyone familar with using this
> >> product, or any other type of diastase or amylase.
> >> Thank you all very much.
> >>
> >> Eileen  Dusek
> >> Central Dupage Hospital
> >>
> >>
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:54:44 -0500
> >From: Richard.Pitman@wri-tr.wmids.nhs.uk (by way of Histonet)
> >Subject: Kevlar Autopsy  Gloves
> >
> >Hi All,
> >
> >Does anyone know a supplier of Kevlar gloves, suitable for use in the
> >autopsy room ? Required to prevent needle stick injuries when sewing
> >bodies. We have chain mail gloves already, but these are obviously no good
> >for this particular task.
> >
> >Thanks,
> >
> >Richard
> >
> >Richard Pitman FIBMS,
> >Head MLSO,
> >Dept of Histology,
> >Worcester Royal Infirmary
> >Great Britain.
> >
> >
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:55:03 -0500
> >From: "Buttigieg Carmen at MOH" <carmen.a.buttigieg@magnet.mt> (by way of
> >Histonet)
> >Subject: RE:Re: h.pylori stain
> >
> >Thanks to all for replying to my plea for an H. pylori stain. I think I'll
> >try
> >out the Tol blue/Alcian yellow for starters. It seems to be the most popular.
> >
> >Thanks again
> >
> >Carmen
> >St. Luke's Hospital
> >Malta
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:55:34 -0500
> >From: Paul Klosen <klosen@neurochem.u-strasbg.fr> (by way of Histonet)
> >Subject: Re: Protein extraction from paraffin blocks
> >
> >At 09:26 11/05/00 -0700, Victoria Baker a ecrit:
> >>Hi All!
> >>
> >>The foundation is looking into extracting proteins
> >>from paraffin/formalin fixed tissue samples.  Thus
> >>far, the success is eh to not so hot.  I was wondering
> >>if anyone had a procedure to do this, currently I
> >>haven't found anything that really gave me an answer.
> >>Given that the protein is masked by fixation, it seems
> >>logical that their has to be a method to unmask it.
> >>In IHC it's digestion, retrieval or both in a
> >>combination.  If anyone has a source I could tap into,
> >>I'd really appreciate it.
> >
> >If you use formalin-fixed tissue, it's quite normal to have some problems.
> >The main problem is that -some of the proteins will be crosslinked, and
> >will thus not migrate at the correct MW in electrophoresis. Also, the
> >cross-linked proteins are difficult to extract. Better results can be
> >obtained using non-additive fixatives like Carnoy or methacarn. I have done
> >this a few years ago using 5% SDS to extract the dewaxed sections. In
> >SDS-PAGE you still have a smear, but many proteins will give an almost
> >correct appearance in Western Blots. If you want to have an idea on the
> >results that can be obtained check Journal of Neurocytology 23: 297-311. We
> >used this to check the specificity of anti-neurofilament monoclonals for
> >different species. As we had some trouble obtaining fresh tissue for
> >extraction, we used our paraffin embedded samples. This approach is also a
> >much better control than using fresh tissue, as the proteins extracted from
> >paraffin sections have gone to a complete histological processing, and are
> >thus somewhat closer to the antigen present in the tissue sections.
> >
> >Paul
> >                                                                         -=-
> >                                                                        (o -)
> >O
> >===============================oOo==(_)==OOo=============================
> >Paul Klosen, PhD
> >CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
> >Universite Louis Pasteur  12, rue de l'Universite
> >F-67000 Strasbourg, FRANCE
> >Tel. 03.88.35.85.04  Fax. 03.88.24.04.61
> >========================klosen@neurochem.u-strasbg.fr========================
> >=
> >S
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:55:55 -0500
> >From: "Buttigieg Carmen at MOH" <carmen.a.buttigieg@magnet.mt> (by way of
> >Histonet)
> >Subject: RE:PAS DIASTASE
> >
> >Dear Eileen
> >
> >We use 1% solution of Diastase from pig pancreas which we buy from BDH.
> > The cat. no. is 39123 3N. We make it up fresh every day.
> >It works perfectly for us.
> >
> >Good luck
> >
> >Carmen
> >St. Luke's
> >Malta
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:56:13 -0500
> >From: "Nader, Alexander" <alexander.nader@wgkk.sozvers.at> (by way of
> >Histonet)
> >Subject: TIA-1 problems
> >
> >We have troubles with the TIA-1 Ab from Coulter. Although our dilution is
> >1:1000, we still have sometimes positivity in reactive T-cells in
> >bone-marrow biopsies.
> >
> >We use the DAKO-Kit as secondary Ab with DAB as chromogen.
> >
> >Dr. Alexander Nader
> >Path. Institut Hanuschkrankenhaus
> >A 1140 Wien, Oesterreich
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:56:33 -0500
> >From: Brown Alex <Alex.Brown@aaaht.scot.nhs.uk> (by way of Histonet)
> >Subject: RE: PAS DIASTASE
> >
> >Hi Eileen,
> >       We use Sigma Amylase ( Type V1-B: from Porcine Pancreas ) We use it
> >as a 1% solution in distilled water for 15mins at room temperature.
> >       Alex Brown
> >       Crosshouse Hospital
> >       Kilmarnock, Scotland
> >
> > ----------
> >From: Eileen_Dusek@cdh.org
> >To: HistoNet@pathology.swmed.edu
> >Subject: PAS DIASTASE
> >Date: Thursday, May 11, 2000 5:21PM
> >
> >Hello everyone,
> >Does anyone have a procedure for Pas Diastase without using saliva.  In
> >this day and age of safety i am surprised we still use our own saliva.
> >I know Sigma has an Amylase Type IX-A, is anyone familar with using this
> >product, or any other type of diastase or amylase.
> >Thank you all very much.
> >
> >Eileen  Dusek
> >Central Dupage Hospital
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:56:54 -0500
> >From: Nora Richards <niznr@unix.ccc.nottingham.ac.uk> (by way of Histonet)
> >Subject: Automated IHS
> >
> >Thanks Sharon for reply - our autoantibody screening by indirect
> >immunofluorescence is a bit different from histochemistry as we need to
> >incubate patients serum with tissue sections as well, so the machines have
> >to be adaptable for this - is your experience only histochemistry or have
> >you ever seen this used for our application?
> >
> >Nora Richards, Immunology dept, Queens Medical Centre, Nottingham
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 13 May 2000 22:57:14 -0500
> >From: "Louise Taylor" <louiset@mail.saimr.wits.ac.za> (by way of Histonet)
> >Subject: RE: Coverglass thickness
> >
> >Hmmm,
> >yes, there are plus factors regarding tape coverslippers BUT.... we have
> >found that after a while the tape peels off, taking with it the section.
> >This is especially serious for teaching and seminar cases  which are used
> >year after year and represent rare and unusual material - so its glass still
> >for all that.  I have also found that there a small irregularities in the
> >tape which also make it difficult when looking at a section for fine details
> >such as ISH signals. One has to keep focussing up and down to correct as one
> >moves along.
> >
> >But generally I agree that there is substantial time saving involved.
> >
> >best regards
> >Louise Taylor
> >
> >
> >- -----Original Message-----
> >From: a i d a n s c h u r r [mailto:Aidan.Schurr@hvh.co.nz]
> >Sent: 11 May 2000 01:48
> >To: Scott Taft; histonet@pathology.swmed.edu
> >Subject: Re: Coverglass thickness
> >
> >
> >>
> >> PS- If you use tape, are you considering switching
> >> back to glass?
> >>
> >> Scott Taft HT(ASCP)
> >> Tucson, AZ
> >>
> >Scott - we use tape, and will *never* go back to glass!  The sheer
> >convenience of the tape system hugely outweighs any loss of
> >optical clarity (which is negligible if your microscope is adjusted
> >correctly).  No more 'blocks' of slides stuck together in the file, no
> >more endless hours fiddling with coverslips and DPX, no more
> >racks of slides drying in ovens, no more cleaning DPX off 40x
> >objectives when a pathologist goes too close to the edge of a wet
> >slide!   oh joy, oh rapture!! (**big grin**)
> >
> >Aidan
> >ps do you get the feeling I rather like this system!?
> >___________________________________________________
> >shin: device for finding furniture in the dark...
> >___________________________________________________
> >a i d a n   c   s c h u r r
> >     mlso,  histology department
> >      hutt valley health
> >       lower hutt, new zealand
> >
> >     ph.  ++64 4 5709173
> >     fax  ++64 4 5709214
> >___________________________________________________
> >
> >
> >
> >
> >----------------------------------------------------------------------
> >
> >Date: 14 May 2000 06:27:17 -0500
> >From: "Muhammad Tahseen" <tahseen@brain.net.pk>
> >Subject: No message ?
> >
> >Hello,
> >I am not getting any message from histonet since last two day?
> >What happened to the list master
> >Thanks
> >Tahseen
> >tahseen@brain.net.pk
> >
> >
> >Here are the messages received yesterday!
> >
> >