Re: Daily Digest

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To:"HistoNet Server" <>, "HistoNet Server" <>
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At 06:24 AM 5/15/00 -0500, HistoNet Server wrote:
Hello Eileen,

I know the saliva is a bit disgusting but it works very well and in a busy 
laboratory I prefer it to weighing out enzymes and waiting for them to warm 
  I recently noticed that when one leaves the control 'PAS only' slide in 
distilled water while digesting out the glycogen in the test  slide  the 
glycogen dissolves out anyway so now I leave it in the oven and only bring 
down to water just as I'm about to add the periodic acid.I tested out 
leaving it in alcohol and this too dissolved out the glycogen.Has anyone 
else observed this.I'm using formalin fixed paraffin embedded 
tissue.Annette Ryan, Drogheda Ireland

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