Re: Citrate pH 1

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From:"J. A. Kiernan" <>
To:Lynn Gardner <>
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Tue, 23 May 2000, Lynn Gardner wrote:

> Could someone please send me a recipe for Citrate buffer pH 1 and pH 10 as
> soon as possible. I have the one for pH 6.0 but would like the others for
> comparison.

   Citrate and citric acid have buffering capacity in the
   range 3.0 to 6.2. There is a phosphate-citrate buffer
   system for the range 2.7 to 7.7.

   pH 1 is not very satisfactory for buffering. There's
   a sodium-chloride-hydrochloric acid mixture and also 
   sodium acetate-HCl, but the buffering capacity is poor.
   For pH 10 use a glycine-NaOH buffer. There's also
   simple 0.01M borax (pH 9+). If you're using the
   buffer boiling hot, its pH won't be the same as it
   is at room temperature.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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