Re: Citrate pH 1
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Lynn Gardner <lynn-gardner@uiowa.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Tue, 23 May 2000, Lynn Gardner wrote:
> Could someone please send me a recipe for Citrate buffer pH 1 and pH 10 as
> soon as possible. I have the one for pH 6.0 but would like the others for
> comparison.
Citrate and citric acid have buffering capacity in the
range 3.0 to 6.2. There is a phosphate-citrate buffer
system for the range 2.7 to 7.7.
pH 1 is not very satisfactory for buffering. There's
a sodium-chloride-hydrochloric acid mixture and also
sodium acetate-HCl, but the buffering capacity is poor.
For pH 10 use a glycine-NaOH buffer. There's also
simple 0.01M borax (pH 9+). If you're using the
buffer boiling hot, its pH won't be the same as it
is at room temperature.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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