Hi,
    I believe Alan jackson has a song that describes the solution to this
problem. "Pop a Top Again". Just push the dropper off and the cap will hold the
rest of the reagent without spilling. We do this for all reagents that have
droppers. Why do they make the darn things like that, I'll never know.
Amos Brooks

"Drew Sally A." wrote:

> We use CAM 5.2 from Becton Dickinson, cat. # 349205...(I'm sure they'd fax
> you a product insert with all the technical info.)...We use Protease 2 for
> 16" on the instrument, 1 drop of antibody, 20" titration...and get very
> strong staining.  For our controls we use breast and colon carcinomas, and
> we also have some cytospins from a met. squam. cell carcinoma...I do have to
> say, however, that the bottle is one of those that is extremely hard to
> squeeze!  Let me know if there's anything else we can help you with on this
> antibody.
>
> Sally Ann Drew-MT(ASCP)
> Univ. of Wis. Hospital and Clinics
> IHC/ISH Clinical/Research Lab
> Madison, WI
> (608)265-6596
> (608)263-1568(fax)
>
> > -----Original Message-----
> > From: Sharon Bledsoe [SMTP:sbledsoe@iupui.edu]
> > Sent: Thursday, May 18, 2000 8:05 AM
> > To:   Drew Sally A.
> > Subject:      RE: CAM 5.2
> >
> > We found the a reference to this antibody listed in a paper, but no
> > further infro.  We'd like to use this as a marker for kidney tissue.
> >
> > I'd appreciate any information you could send me.
> >
> > thanks,
> > Sharon
> >
> >
> > >What kind of info do you need?  If you'd like to know vendor, protocol,
> > >control tissue, etc. that we use, just tell me and I'll list it all for
> > you.
> > >We use Ventana ES and genII...
> > >
> > >Sally Ann Drew-MT(ASCP)
> > >Univ. of Wis. Hospital and Clinics
> > >IHC/ISH Clinical/Research Lab
> > >Madison, WI
> > >(608)265-6596
> > >(608)263-1568(fax)
> > >
> > >  > -----Original Message-----
> > >  > From:    Sharon Bledsoe [SMTP:sbledsoe@iupui.edu]
> > >  > Sent:    Wednesday, May 17, 2000 4:24 PM
> > >  > To:      histonet@pathology.swmed.edu
> > >  > Subject: CAM 5.2
> > >  >
> > >  > Can anyone give me any information of CAM 5.2 antibody?
> > >  >
> > >  > Thanks