Re: Bone Marrow Trephines

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From:Bro Lauren Ball <>
Content-Type:text/plain; charset="iso-8859-1"

    I didn't think it was possible to over decal in EDTA.  RDO yes, any of
the acid yes.  I've used EDTA and EDTA tetrasodium salt, and mixtures of
these.  I find EDTA to slow for routine work.  Bone marrows can take up to
24 hours.  But they look great.  RDO only takes 15 minutes.
----- Original Message -----
From: Buttigieg Carmen at MOH <>
To: <>
Sent: Monday, May 29, 2000 6:00 AM
Subject: Bone Marrow Trephines

> Dear Histonetters
> I need your help!
> We routinely receive bone marrow trephines which are quite boney. We
> decalcify in EDTA but sometimes the endpoint is overshot and the cellular
> structures are ruined. We do not have a resin set-up, we process
everything to
> paraffin. We do not have an x-ray set-up either.
> Is there any way to cut a paraffin embedded bone marrow trephine on a
> rotary microtome without having to decalcify it?
> Thanks.
> Carmen
> St. Luke's Hospital
> Malta

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