Re: Bone Marrow Trephines
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From: | a i d a n s c h u r r <Aidan.Schurr@hvh.co.nz> |
To: | "t.hacker@har.mrc.ac.uk" <T.Hacker@har.mrc.ac.uk>, carmen.a.buttigieg@magnet.mt, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
My guess would be that the trephines are not being adequately
fixed before being decalcified. This would give the effect you
describe.
Aidan
> Date sent: Mon, 29 May 2000 14:00:00 +0100
> From: Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt>
> Subject: Bone Marrow Trephines
> To: histonet@pathology.swmed.edu
>
> > Dear Histonetters
> >
> > I need your help!
> >
> > We routinely receive bone marrow trephines which are quite boney. We usually
> > decalcify in EDTA but sometimes the endpoint is overshot and the cellular
> > structures are ruined. We do not have a resin set-up, we process everything to
> > paraffin. We do not have an x-ray set-up either.
>
>
> Carmen,
> I am surprised that your EDTA is giving you problems with over-
> decalcification (check your recipe). The whole point of using this
> chelating agent is that it is gentle and controllable, ideal for
> research but not if you are in a hurry. I have left boney tissue in
> EDTA for 8-10 weeks with no effect on subsequent staining. When
> I used to look at BM trephines we would bisect, half into 10%formic
> acid in 10% sodium citrate for 24-48 hours then into paraffin, the
> other half into resin (undecalcified).
> Terry.
>
___________________________________________________
shin: device for finding furniture in the dark...
___________________________________________________
a i d a n c s c h u r r
mlso, histology department
hutt valley health
lower hutt, new zealand
ph. ++64 4 5709173
fax ++64 4 5709214
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