Re: Bone Marrow Trephines

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From:a i d a n s c h u r r <>
To:"" <>,,
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My guess would be that the trephines are not being adequately 
fixed before being decalcified.  This would give the effect you 


> Date sent:      	Mon, 29 May 2000 14:00:00 +0100
> From:           	Buttigieg Carmen at MOH <>
> Subject:        	Bone Marrow Trephines
> To:    
> > Dear Histonetters
> > 
> > I need your help!
> > 
> > We routinely receive bone marrow trephines which are quite boney. We usually 
> > decalcify in EDTA but sometimes the endpoint is overshot and the cellular 
> > structures are ruined. We do not have a resin set-up, we process everything to 
> > paraffin. We do not have an x-ray set-up either.
> Carmen,
>  I am surprised that your EDTA is giving you problems with over-
> decalcification (check your recipe). The whole point of using this 
> chelating agent is that it is gentle and controllable, ideal for 
> research but not if you are in a hurry. I have left boney tissue in 
> EDTA for 8-10 weeks with no effect on subsequent staining. When 
> I used to look at BM trephines we would bisect, half into 10%formic 
> acid in 10% sodium citrate for 24-48 hours then into paraffin, the 
> other half into resin (undecalcified).
> Terry.

shin: device for finding furniture in the dark...
a i d a n   c   s c h u r r 
     mlso,  histology department
      hutt valley health
       lower hutt, new zealand

     ph.  ++64 4 5709173
     fax  ++64 4 5709214

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