Re: Bone Marrow Trephines
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From: | "t.hacker@har.mrc.ac.uk" <T.Hacker@har.mrc.ac.uk> |
To: | Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt> |
Reply-To: | |
Content-Type: | |
Date sent: Mon, 29 May 2000 14:00:00 +0100
From: Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt>
Subject: Bone Marrow Trephines
To: histonet@pathology.swmed.edu
> Dear Histonetters
>
> I need your help!
>
> We routinely receive bone marrow trephines which are quite boney. We usually
> decalcify in EDTA but sometimes the endpoint is overshot and the cellular
> structures are ruined. We do not have a resin set-up, we process everything to
> paraffin. We do not have an x-ray set-up either.
Carmen,
I am surprised that your EDTA is giving you problems with over-
decalcification (check your recipe). The whole point of using this
chelating agent is that it is gentle and controllable, ideal for
research but not if you are in a hurry. I have left boney tissue in
EDTA for 8-10 weeks with no effect on subsequent staining. When
I used to look at BM trephines we would bisect, half into 10%formic
acid in 10% sodium citrate for 24-48 hours then into paraffin, the
other half into resin (undecalcified).
Terry.
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