I have used Mollifex in another lab, long ago.  Now we just use a soap
solution and get good results.

Jennifer MacDonald

> ----------
> From: 	Barry Rittman[SMTP:brittman@mail.db.uth.tmc.edu]
> Sent: 	Wednesday, May 24, 2000 1:10 PM
> To: 	histology
> Subject: 	Re: softening tendon
> 
> Rita,
> what you need is "Mollifex" sold by BDH
> barry
> 
> Rita Angel wrote:
> 
> > Histonetters,
> >
> > I recently read in a histology book that there are procedures before
> > processing tendon or other hard tissue, that will soften these tissues
> and
> > greatly improve the sections you get when cutting.  The book did not
> give
> > this protocol, but mentioned using glycerol, alcohol and aniline oil
> > mixtures.  It also mentionened a brand name of "Millifex".  Does anyone
> > have a protocol for this type of tissue?  We are sectioning rabbit
> tendon
> > and mouse tendon at this time.  We are just soaking longer in an
> ice/water
> > mixture, but I would like to get better sections if possible.
> > Thank you for your help.
> >
> > Rita Angel
> 
>