RE: heart tissue processing
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| From: | Cynthia Favara <cfavara@niaid.nih.gov> |
| To: | "'kwalters'" <kwalters@blue.weeg.uiowa.edu>, histo <histonet@pathology.swmed.edu> |
| Reply-To: | |
| Content-Type: | text/plain; charset="iso-8859-1" |
Kathy,
I have done heart from sheep, mice, rabbits and guinea pigs. Usually
fix for 24hr minimum. Use graded ETOH for 45 minutes each station as follows
70%, 80%, 95%. 2x100%, 3xPropar[Anatech], 2x infiltrating mediium[surgipath]
30 minutes each, 2x embedding mediun[surgipath]. Heart has never been as
much of a problem as brain or spleen from mice. Good Luck!!!
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286
e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov>
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