RE: coverslipping problem

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From:Brown Alex <> (by way of Histonet)
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Thankfully we've never had this problem Shar.
We use xylene as a clearing agent and DPX as a mountant ( from BDH ). We're
using a Leica CV5000 coversliper with the 'Mountant Type' set to 3 and the
'Mountant Volume' set to 3. We also use Shandon coverslips 22 x 50's
	Hope this helps
		Alex Brown
		Crosshouse Hospital
		Kilmarnock, Scotland
From: sharon lang
Subject: coverslipping problem
Date: Tuesday, May 16, 2000 4:50PM

Dear Fellow Histonetters:
I have been running into some coverslipping issues regarding my glass
coverslipper.   I have tried a number of mountants and solvents without yet
I still have coverslips that are falling off (not as bad as the previous
tape coverslipper)about 30% of the time.  For all those of you who own a
glass coverslipper what type of mountant and clearing agent do you use?
Have you experienced or seen this before?
Thanks in advance!
The Cutting Edge
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