RE: coverslipping (DPX etc)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of Histonet) |
To: | HistoNet@pathology.swmed.edu |
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Content-Type: | text/plain; charset="us-ascii" |
On Wed, 17 May 2000, Brown Alex wrote:
> We use xylene as a clearing agent and DPX as a mountant ( from BDH ).
Is DPX from BDH now OK? If so, this is Very Good News.
Some years ago BDH's DPX "went off" and contained transparent but
visible droplets that rendered it useless. Our BDH agent (VWR)
recommended Entellan (from Merck) instead, and at a much higher price.
Entellan is an excellent mounting medium, but it is (according to
the label) mostly poly(methyl methacrylate), and not polystyrene (80kD),
which was the principal ingredient of DPX. The two Cytoseal media are
also poly(methyl methacrylate)-based, are cheaper than Entellan, and
come in handy squeezy bottles - no need for a glass rod to dispense
the drops. The Cytoseals aren't quite as good as Entellan, however,
because tiny bubbles often form near the edges of the coverslips
while the slides are drying. This doesn't look nice but it doesn't
often intrude onto a centrally placed section or smear. But it
seems to be a shortcoming of Cytoseal that never happens with the
more expensive Entellan and was never seen with DPX.
If someone from the firm that makes Cytoseal reads this, perhaps
we'll get some advice about avoiding the peripheral bubbles.
Back to DPX.
The abbreviation DPX is from Distrene-80, a trade-name of the polymer,
Plasticizer, which could be a cresyl phosphate or dibutyl phthalate,
and Xylene, the excipient.)
The great thing about DPX is that you can, in principle, make it
yourself from published ingredients. Unfortunately, one of these is the
80 kD polystyrene, which may not always be exactly the same as the
Distrene-80 of the 1940s and '50s.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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