Hi Eileen,
Better late than never :)  We also use Sigma Type V1-B and we use approx 50
mg in 50 ml of tap water at room temp for 20 minutes.  It is very
inexpensive and always works.  
Good Luck.

Mickie

Michael L Johnson, BS, HTL(ASCP)
Histology Supervisor
Department of Pathology
Sacred Heart Medical Center
W. 101 8th Avenue
Spokane, WA 99220

johnsom@shmc.org


-----Original Message-----
From: Eileen_Dusek@cdh.org [mailto:Eileen_Dusek@cdh.org]
Sent: Thursday, May 11, 2000 9:21 AM
To: HistoNet@pathology.swmed.edu
Subject: PAS DIASTASE


Hello everyone,
Does anyone have a procedure for Pas Diastase without using saliva.  In
this day and age of safety i am surprised we still use our own saliva.
I know Sigma has an Amylase Type IX-A, is anyone familar with using this
product, or any other type of diastase or amylase.
Thank you all very much.

Eileen  Dusek
Central Dupage Hospital